Regulatory

Part:BBa_K309001:Design

Designed by: Chris Palmer   Group: iGEM10_Queens-Canada   (2010-10-22)


pSra-10 (C. elegans promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 270
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1849
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1142


Design Notes

The exact length and sequence of the functional sequence that acts as the promoter in C. elegans has not been elucidated. Thus this sequence is a section of genomic DNA that exists in front of the Sra-10 gene that has been previously demonstrated to function as a promoter, with the same expression pattern as the Sra-10 gene.


Source

From C. elegans genomic DNA.

References